Staining:Article Title: Analysis of changes in high‐mobility group box 1, receptor for advanced glycation endproducts, and T helper 17/regulatory T balance in severe preeclampsia with acute heart failure
Article Snippet: .. A Human Th17 staining kit (no: KTH117) and a human Treg staining kit (no: KTR101) were procured from Hangzhou Lianke Biotech Co., Ltd. RPMI‐1640 medium (catalog no.: 10491) was purchased from Beijing Solarbio Science & Technology Co., Ltd. HMGB1 (no.: MB‐3547A), RAGE (no.: MB‐1419A), IL‐17 (no.: MB‐0180A), IL‐6 (no.: MB‐0049A), TGF‐β (no.: MB‐3393A), IL‐10 (no.: MB‐3705A), and NT‐proBNP (no.: MB‐3940A) were detected using an enzyme‐linked immunosorbent assay (ELISA), and the respective ELISA kits were purchased from Jiangsu Meibiao Biotechnology Co., Ltd. A TDL‐40C low‐speed desktop large‐capacity centrifuge was purchased from Shanghai Anting Scientific Instrument Factory. .. A NovoCyte flow cytometer was purchased from ACEA Biosciences, USA, and a Multiskan Spectrum 1500 microplate reader was purchased from Thermo Scientific, Germany.
Article Title: Antagonising Yin Yang 1 ameliorates the symptoms of lupus nephritis via modulating T lymphocyte signaling.
Article Snippet: A single-cell suspension was prepared from isolated spleens, followed by staining with appropriate antibodies (anti-mouse CD3-BV510, anti-mouse CD4-FITC, anti-mouse CD19-PE, anti-mouse CD8α-APC-Cy7, Biolegend, USA; mouse Th17 staining kit, Multi sciences, China). .. Alternatively, peripheral blood mononuclear cells (PBMCs) were obtained and stained with a human Th17 staining kit (Multi sciences, China). .. Staining was performed in the dark at room temperature, analyzing 10,000 cells/sample with a BD LSRFortessa X-20.
Article Title: Analysis of changes in high-mobility group box 1, receptor for advanced glycation endproducts, and T helper 17/regulatory T balance in severe preeclampsia with acute heart failure.
Article Snippet: .. A Human Th17 staining kit (no: KTH117) and a human Treg staining kit (no: KTR101) were procured from Hangzhou Lianke Biotech Co., Ltd. RPMI-1640medium (catalog no.: 10491) was purchased fromBeijing Solarbio Science & Technology Co., Ltd. HMGB1 (no.: MB-3547A), RAGE (no.: MB-1419A), IL-17 (no.: MB-0180A), IL-6 (no.: MB-0049A), TGF-β (no.: MB-3393A), IL-10 (no.: MB-3705A), and NT-proBNP (no.: MB-3940A) were detected using an enzyme-linked immunosorbent assay (ELISA), and the respective ELISA kits were purchased from Jiangsu Meibiao Biotechnology Co., Ltd. A TDL-40C low-speed desktop large-capacity centrifuge was purchased from Shanghai Anting Scientific Instrument Factory. .. A NovoCyte flow cytometer was purchased from ACEA Biosciences, USA, and a Multiskan Spectrum 1500 microplate reader was purchased from Thermo Scientific, Germany.
Enzyme-linked Immunosorbent Assay:Article Title: Analysis of changes in high‐mobility group box 1, receptor for advanced glycation endproducts, and T helper 17/regulatory T balance in severe preeclampsia with acute heart failure
Article Snippet: .. A Human Th17 staining kit (no: KTH117) and a human Treg staining kit (no: KTR101) were procured from Hangzhou Lianke Biotech Co., Ltd. RPMI‐1640 medium (catalog no.: 10491) was purchased from Beijing Solarbio Science & Technology Co., Ltd. HMGB1 (no.: MB‐3547A), RAGE (no.: MB‐1419A), IL‐17 (no.: MB‐0180A), IL‐6 (no.: MB‐0049A), TGF‐β (no.: MB‐3393A), IL‐10 (no.: MB‐3705A), and NT‐proBNP (no.: MB‐3940A) were detected using an enzyme‐linked immunosorbent assay (ELISA), and the respective ELISA kits were purchased from Jiangsu Meibiao Biotechnology Co., Ltd. A TDL‐40C low‐speed desktop large‐capacity centrifuge was purchased from Shanghai Anting Scientific Instrument Factory. .. A NovoCyte flow cytometer was purchased from ACEA Biosciences, USA, and a Multiskan Spectrum 1500 microplate reader was purchased from Thermo Scientific, Germany.
Article Title: Analysis of changes in high-mobility group box 1, receptor for advanced glycation endproducts, and T helper 17/regulatory T balance in severe preeclampsia with acute heart failure.
Article Snippet: .. A Human Th17 staining kit (no: KTH117) and a human Treg staining kit (no: KTR101) were procured from Hangzhou Lianke Biotech Co., Ltd. RPMI-1640medium (catalog no.: 10491) was purchased fromBeijing Solarbio Science & Technology Co., Ltd. HMGB1 (no.: MB-3547A), RAGE (no.: MB-1419A), IL-17 (no.: MB-0180A), IL-6 (no.: MB-0049A), TGF-β (no.: MB-3393A), IL-10 (no.: MB-3705A), and NT-proBNP (no.: MB-3940A) were detected using an enzyme-linked immunosorbent assay (ELISA), and the respective ELISA kits were purchased from Jiangsu Meibiao Biotechnology Co., Ltd. A TDL-40C low-speed desktop large-capacity centrifuge was purchased from Shanghai Anting Scientific Instrument Factory. .. A NovoCyte flow cytometer was purchased from ACEA Biosciences, USA, and a Multiskan Spectrum 1500 microplate reader was purchased from Thermo Scientific, Germany.
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